CLASS XII (THEORY)
One paper Time:3Hours Total Marks: 70 (180 Periods) Unit V: Protein and Gene Manipulation Marks 40 (100 Periods) Chapter I: Recombinant DNA Technology 15 Marks (40 Periods)
Introduction
Tool of rDNA Technology
Making rDNA
Introduction of Recombinant DNA into Host Cells
Identification of Recombinants Polymerase ChainReaction (PCR) Hybridization Techniques
DNA Library
DNA Sequencing
Site-directed Mutagenesis
Chapter II: Protein Structure and Engineering 15Marks (35 Periods)
Introduction to the World of Proteins
3-D Shape of Proteins
Structure-Function Relationship in Proteins
Purification of Proteins Characterization of Proteins Protein Based Products
Designing Proteins(Protein Engineering)
Chapter III: Genomicsand Bioinformatics 10 Marks (25 Periods)
Introduction
Genome Sequencing Projects
Gene Predictionand Counting
Genome Similarity, SNPs and Comparative Genomics
Functional Genomics
Proteomics
History of Bioinformatics Sequences and Nomenclature Information Sources
Analysis using Bioinformatics Tools
Unit VI : Cell Cultureand Genetic Manipulation 30 Marks (80 Periods)
Chapter I: Microbial Culture and Applications 10Marks (26 Periods)
Introduction
Microbial Culture Techniques
Measurement and Kinetics of Microbial Growth
Scale up of MicrobialProcess Isolationof Microbial Products Strain isolation and Improvement
Applications of Microbial Culture Technology
Biosafety Issues in Microbial Technology
Chapter II: Plant Cell Culture and Applications 10Marks (27 Periods)
Introduction
Cell and Tissue Culture Techniques Applications of Cell and Tissue Culture Gene TransferMethods in Plants Transgenic Plantswith Beneficial Traits Biosafety in Plant Genetic Engineering
Chapter III: Animal Cell Culture and Applications 10 Marks (27 Periods)
Introduction
Animal Cell CultureTechniques Characterisation of Cell Lines Methods of Gene Delivery into Cells Scale-up of AnimalCulture Process Applications of Animal Cell Culture Stem Cell Technology
Tissue Engineering
Practicals
(60 Periods)
Note: Every student will be required to do the following experiments during the academic session
List of Experiments
1. Isolation of bacterialplasmid DNA and its detection by gel electrophoresis
2. Restriction digestionof plasmid DNA and its analysis by gel electrophoresis
3. Bacterial transformation using any plasmid
4. Data retreivaland data base search using internet site NCBI
5. Download a DNA and protein sequence from internet, analyse it and comment on it.
6. Cell viabilityassay
7. Determination of blood groups
8. Estimation of DNA
9. Ion-exchange chromatography for proteins.
10. Reading of DNA sequencinggel to arrive at the sequence
11. Estimation of blood glucose by enzymatic method (GOD/POD)
12. Project work
Scheme of Evaluation:
Time: 3 Hours Max. Marks 30
The scheme of evaluation at the end of the session will be as under:
A. Two experiments : 6+6 (onlyone computer based practical) Practical record : 04
Viva on Practicals : 04
B. Project work :
Write up : 05
Viva on project : 05
Total 30
Recommended Books:
1. A Text Book of Biotechnology - Class XI : Published by CBSE, New Delhi
2. A Laboratory Manual of Biotechnology -Class XI : Publishedby CBSE, New Delhi
3. A Text Book of Biotechnology - Class XII : Published by CBSE, New Delhi
4. A Laboratory Manual of Biotechnology -Class XII : Publishedby CBSE, New Delhi
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